Dear Li, Since SU-8 is transparent, you need to align the mask to alignment marks on your wafer. In general, you don't want to develop the first layer of SU8 because the resulting topology will make it difficult to spin-coat the second layer of SU8. For more information on multilayer soft lithography, I would recommend Tien et al, PNAS, Feb. 2002. Darren Gray Graduate Student Johns Hopkins University Hi all, I^Òm working on 2-level PDMS structure fabrication so that I should spin SU-8 twice. But I met a problem when I align the two layers. Since the two layers are both SU-8 and SU-8 seemed transparent, it really hard to observe the alignment mark under the microscope during the alignment process. My first layer is about 7um, second layer is 50um. Did anybody met this problem? Any ideas about this? Another issue is that according to some papers from Whitesides group, they said the first SU-8 should not be developed. I couldn't find the reason why the first layer should not be developed. Is there anybody can give me hints for this issue? I tried developed and undeveloped the first layer. Developed can be a little bit better, but still not enough to clearly observe under microscope. Thank for your help. Li Wang 412-2682514 Carnegie Mellon University