Hi Sameer,

   I can suggest you a few techniques to measure your deflection based on the
number of molecules deposited.

 1. Use can use AFM techniques to measure the deflection. This is one common
technique. I believe you may need to make some modifications to the AFM setup to
measure these changes.

 2. If you have fabricated the cantilevers yourself try making piezoresistive
cantilevers with a direct read out circuit. I have a few papers on this which
may help you.

 The above techniques are not probabilistic the sensitivity in the above two
cases is directly proportional to the number of molecules and it also depends on
your design of the cantilevers.

  Regarding how to coat different cantilevers with different antibodies what you
could possibly do is to mask but am not pretty sure. Can tell you about this in
a week.

 Hope this helps.

Regards,

Rakesh Katragadda
Graduate Researcher
Wayne State University
Ph: (313)577-4933




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