Santanu, Antibodies generally do not bind on their own to a bare Au surface; you will need a linker. I suggest this paper: @Article{thiol-protocol, author = {Nikin Patel and Martyn C. Davies and Mark Hartshorne and Richard J. Heaton and Clive J. Roberts and Saul J. B. Tendler and Philip M. Williams }, title = {Immobilization of Protein Molecules onto Homogeneous and Mixed Carboxylate-Terminated Self-Assembled Monolayers}, journal = {Langmuir}, year = 1997, volume = 13, number = 24, pages = {6485-90} }