Dear Hongjun we use the "air plasma " to activate the PDMS for bonding. We just evapoarate the reaction chamber of an ordinary plasma cleaner (Harrick) to the appropriate pressure and expose the PDMS surface to the plasma obtained in the residual air atmosphere . We apply the plasma only about 2 minutes to obtain a thin sio2 layer on the pdms surface. You should not produce a too thick layer which actually embrittles the pdms at the surface and does not seal anymore. You should apply some slight pressure to contact the pdms and seal it but thats of course very dependent on your structure that should be distroted to much by the pressure. Hans-Georg Braun