We mix a rhodamine tag in a molar ratio of 1:200 rhodamine:solvent. -Mike >>> dagarshali@gmail.com 08/04/05 12:07 PM >>> Hi, I am trying to pass a liquid with a flourescent dye(rhodamine 6g) to get some images using confocal microscopy. But before doing that I need to find the right concentration of rhodamine. To do that I am using a Xenon lamp with a PIV filter. Both these are parts of an inverted Nikon microscope. I would like get some info on Rhodamine 6G. Some literature that I can refer to and also some starting concentration to work with.