Hi All, I am currently working on fabricating microfluidic channels on coverglass using SU8-2025 and using the following protocol: 1. Spin SU8 2025 on No. 1 coverglass. 500 rpm -> 2000 rpm. Coverglass was blown with nitrogen before spin coating. 2. Pre-bake. 65 degree C for 1 min and 95 degree C for 3 min. 3. Exposure. 1 min @ 4.5 mW/cm^2 4. Post Bake 65 degree C for 1 min and 95 degree C for 3 min. 5. Development SU8 developer. No IPA rinsing. At first, the feature came out nicely, but recently, the success rate dropped aggressively. I could see bright edges along the channels and sometimes it is really difficult to develop out the un-crosslinked polymer in the un-exposed area. It seems that the resist film in unexposed area is shattered into small pieces. If you could kindly have a look at the following image (http://base.google.com/base/items?oid=10375407859124322045), you may find the Y shape channel (width 200 micron) surrounded by some colorful bright edges. I suspect this bright edge is due to the peel-off of SU8 from coverglass, but cannot figure out why it happens. What puzzles me most is that by the same procedure, I can obtain perfect channel if lucky enough. Unfortunately, I don't know what "luck" means here. So the result changes from trial to trial without an obvious consistent pattern. For example, I tried 10 samples today. The first two were perfect. But the following were worse and worse. For the last one, it takes barely 10 second to develop such edges after I started development, but the unexposed resist wasn't desolved even after 1 min. I tried different combination of process configuration before and it seems that the above protocol is the best for me. But, as the result is not reproducible, I doubt ... I would appreciate it very much if any of you could offer me some hint on this issue and thanks for reading this message. Best, Liu Yu