Liu, I suspect you need to wash your glass. There are a lot of proceedures that you could use. Perhaps RCA (SC1), or a hot soapy alkaline wash (many are commecially available, for example Contrad 70). Glass cleaning can be quite challenging but if you are not doing anything right now it may not take much to make a big difference. good luck, Dwayne yul.fany@gmail.com wrote: Hi All, I am currently working on fabricating microfluidic channels on coverglass using SU8-2025 and using the following protocol: 1. Spin SU8 2025 on No. 1 coverglass. 500 rpm -> 2000 rpm. Coverglass was blown with nitrogen before spin coating. 2. Pre-bake. 65 degree C for 1 min and 95 degree C for 3 min. 3. Exposure. 1 min @ 4.5 mW/cm^2 4. Post Bake 65 degree C for 1 min and 95 degree C for 3 min. 5. Development SU8 developer. No IPA rinsing. At first, the feature came out nicely, but recently, the success rate dropped aggressively. I could see bright edges along the channels and sometimes it is really difficult to develop out the un-crosslinked polymer in the un-exposed area. It seems that the resist film in unexposed area is shattered into small pieces. If you could kindly have a look at the following image (http://base.google.com/base/items?oid=10375407859124322045), you may find the Y shape channel (width 200 micron) surrounded by some colorful bright edges. I suspect this bright edge is due to the peel-off of SU8 from coverglass, but cannot figure out why it happens. What puzzles me most is that by the same procedure, I can obtain perfect channel if lucky enough. Unfortunately, I don't know what "luck" means here. So the result changes from trial to trial without an obvious consistent pattern. For example, I tried 10 samples today. The first two were perfect. But the following were worse and worse. For the last one, it takes barely 10 second to develop such edges after I started development, but the unexposed resist wasn't desolved even after 1 min. I tried different combination of process configuration before and it seems that the above protocol is the best for me. But, as the result is not reproducible, I doubt ... I would appreciate it very much if any of you could offer me some hint on this issue and thanks for reading this message. Best, Liu Yu