Hi All, I am making a microfluidic device for electrokinetic studies. The device will consist of a channel deep-etched into silicon and a top layer consisting of glass. I want to use anodic bonding to bond the device. Because I will use the device for electrokinetic studies I need to be able to apply high voltages over the liquid in the channel. A problem here is of course that the native oxide on the silicon wafer will breakdown causing a short circuit through the silicon wafer. Therefore I want to apply an insulation layer on the silicon before the bonding. This layer will most likely be an oxide. The insulating layer is likely to interfere with the anodic bonding process. Has anyone tried a similar process ? The parameters that I am most interested in are the thickness of the insulating layer and the voltage and temperature for the anodic bonding process.... Voltages that I expect to use are around 50 - 250V. Any help is greatly appreciated ! :-) Heiko van der Linden Delft University of Technology Delft, the Netherlands