Hi The easiest will be to oxidize the Si and then polish the surface. If you don't have access to polish, you can use resist in the channels and etch but this is a bit tricky shay -----Original Message----- From: mems-talk-bounces@memsnet.org [mailto:mems-talk-bounces@memsnet.org] On Behalf Of H.J. van der Linden Sent: Monday, July 09, 2007 5:29 PM To: General MEMS discussion Subject: [mems-talk] anodic bonding and passivation layer thickness Hi All, I am making a microfluidic device for electrokinetic studies. The device will consist of a channel deep-etched into silicon and a top layer consisting of glass. I want to use anodic bonding to bond the device. Because I will use the device for electrokinetic studies I need to be able to apply high voltages over the liquid in the channel. A problem here is of course that the native oxide on the silicon wafer will breakdown causing a short circuit through the silicon wafer. Therefore I want to apply an insulation layer on the silicon before the bonding. This layer will most likely be an oxide. The insulating layer is likely to interfere with the anodic bonding process. Has anyone tried a similar process ? The parameters that I am most interested in are the thickness of the insulating layer and the voltage and temperature for the anodic bonding process.... Voltages that I expect to use are around 50 - 250V.