Start with 0.01% Tween-20 by volume (e.g., 100 uL Tween-20 in 1 L of water). According to a paper I've seen, concentrations greater than this do not lower the surface tension, while concentrations lower than this result in higher surface tension. From experience 0.01% is nice enough to work with, while 0.1% produces more foaming. --Kirt Williams ----- Original Message ----- From: "Vishwa"To: "General MEMS discussion" Sent: Thursday, February 21, 2008 9:24 AM Subject: [mems-talk] How much Tween 20 to add > Hi, > I am running some microPIV expts in a channel, the dimensions of which are > 50mm * 2.5mm* 45um. There ends up being a lot of bubble formation in the > channel and I was advised to use some surfactant like Tween 20. I bought a > bottle of that. But it doesn't come with any guidelines and to how much > should be added. I would appreciate if you could tell me some number as a > safe starting point. > > The flow tracers that I use are carboxylated polystyrene beads in nanopure > water.