Gareth, thanks for your reply, that is true I have not use any filters during exposure, I think a good filter which cut off the UV light below 350 will help. And thank you Eowin, thanks for your suggestion, but I want to make a PDMS channels which may bond with a ITO glass substrate, and I use a silicon waffer as a sub for SU-8 PR, the silicon waffer is opaque one and difficult to observe under a microscope. I think I should try a filter. 2008/10/29 eowin rohan> Hello Liang, > > I think Gareth is right, it looks like a lot of thickness (50um) and too > much short development time, especially if you take into account the sieves > dimensions (around 20um). It will be difficult develop the unsposed parts... > or perhaps impossible at all: You have light scattering so it is possible > that at any depth some photons are reaching the SU-8 under de sieve mask, so > could be polymerised and so it will very difficult develop it. > > I really don´t understand exactly your channel-sieve sistem, especially the > arc-sieve geometry, but looks like something like a block with "holes" with > arc shape. You want the SU-8 just as a mould to get the chanel with "walls" > with arc shape in PDMS. You can´t get holes from one side to other in su-8, > so your walls in PDMS are not so high as you want, and cells flow away > freely, isn´t it?Well, why not make the channel directly in SU-8? The only > thing you will need is invert the mask, leaving the sives clear and the > channel dark. I hope this idea works well for you. > > regards > > Eowin -- 赵亮 南京大学化学化工学院 Liang Zhao School of Chemistry and Chemical Engineering, Nanjing University