I think you can try to use fluorophore labeled micro beads. The porosity
of PDMS is often much smaller than the diameter of these beads.


On Sun, 20 Sep 2009 09:03:00 +0900, 김태진  wrote:
> Hi all,
>
> I've been trying to flow in fluorescence die (Rhodamine) in a PDMS
> microfluidic channel to do some flow characterizations.
>
> The problem is, the vicinity of the pdms channel became bright as well
> after about 5 minutes.
>
> It almost seemed like that the fluoresent die was diffused into the pdms.
>
> Is there a way to solve this problem?
>
> Thanks!
>
> TJ

Peng Li
Research Assistant
Department of Mechanical Engineering & Applied Mechanics
University of Rhode Island
133D Kirk, 92 Upper College Road,
Kingston, RI  02881
Phone: (401) 874-2247