Hi TJ, BSA, bovine serum albumin, can block some porous matrix of PDMS. By incubation concentrated BSA in channels overnight, adsorbed/absorbed Rhodamine can be reduced. HC On Sat, Sep 19, 2009 at 8:03 PM, 김태진wrote: > Hi all, > > I've been trying to flow in fluorescence die (Rhodamine) in a PDMS > microfluidic channel to do some flow characterizations. > > The problem is, the vicinity of the pdms channel became bright as well > after about 5 minutes. > > It almost seemed like that the fluoresent die was diffused into the pdms. > > Is there a way to solve this problem? > > Thanks! > > TJ Research Assistant, Mech. Eng., URI 92 Upper College Rd., Kingston, RI 02881 Office Tel : 401-874-2247