Hi TJ, Do you bond your PDMS to a glass slide? If you do your microscopy through the glass, a solution could be to make black PDMS - in this case the rhodamine diffusing into the PDMS won't bother you so much. To make black PDMS, just add ~1g of black printer toner per 50-60 g of PDMS during mixing. Good luck! Regards, Dirk Renckens TUDelft Chem Eng PhD candidate -----Original Message----- From: 김태진 [mailto:tjnumber1@hotmail.com] Sent: 20 September 2009 02:03 To: mems-talk@memsnet.org Subject: [mems-talk] flowing in rhodamine in pdms channel Hi all, I've been trying to flow in fluorescence die (Rhodamine) in a PDMS microfluidic channel to do some flow characterizations. The problem is, the vicinity of the pdms channel became bright as well after about 5 minutes. It almost seemed like that the fluoresent die was diffused into the pdms. Is there a way to solve this problem? Thanks! TJ