Hi Goucheng, you have some options to solve the problem: First option: - You always can do the master SU-8 in a negative way, simply inverting the mask design (use a negative of your normal mask) In this way you get a right SU-8 master, so a simple soft-litho will give you the right PDMS replica. Second option:- What if you cannot get a negative mask?? You simply will need a few more steps: 1-Use your normal mask and get the normal SU-8 master. 2-Soft-litho: to obtain the inverse PDMS replica. 3-Use the PDMS replica as a mold and pour fresh SU-8 on it, then cure it with UV 4-Peel the SU-8 replica, this is the right replica- the same result you will get with a inverse mask. (Peeling should be easy, due to you have a PDMS stamp) 5-Soft-litho of the SU-8 replica to obtain your desired PDMS estructure... and you do it. it works fine to me. Regards Eowin