On Wed, 10 Mar 1999, Sung Jin wrote:

>
> My problem is that I do not know what kind of liquid does that.
> If anybody knows the answer, please help me.
> I would appreciate knowing the commercial name of the agent,
> and if there is any special procedures to use it (is it mixed
> in water?  or alcohol?).
        You can use a green dye called floroscene, which florosces in
bluish green wavelength.(650nm). It is mixible in ethanol and also in
water. ( I don't remember the solubilities.) Special precaution is that
you need to expose the dye to white light, for atleast 1 hr. before you
get appreciable floroscence intensity for about fifteen to twenty minutes.
This dye is available with Molecular Probes Inc.,MERK, ALDRICH, SIGMA
corp. These companies also have other dyes which are costlier but
give out different wavelengths as required, you may request their
catalogues from their webpages.

Good Luck
Amit



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Amit Shiwalkar                         Dept. Of Bio-Medical Engineering.
3,Vasant,                              IIT Bombay.
Carter Road,                           Powai, Bombay-400076
Khar,
Bombay(Mumbai)-400052
INDIA.


                     Email: amits@cc.iitb.ernet.in

" Reality Is a Figment of IMAGINATION "
                                ----------- Amit Shiwalkar

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