I'm investigating the suitability of SU-8 for structures in an experimental setup I'm working on, and I have a couple of concerns that I'd like to look into. I'm most worried about fluorescence behavior of SU-8. I'd like to be able to use fluorescence microscopy to visualize fluorescently-labeled protein bundles suspended on SU-8 pillars (fabricated on a glass cover slip). I have flexibility in my choice of fluorophores, so it would seem wise to avoid those with excitation frequencies in the UV range that SU-8 absorbs. But I'm concerned about slight absorption and emission of wavelengths above 400nm that aren't a problem for most uses but could get in my way. Is anyone aware of info on absorption and emission spectra of SU-8 after it is baked & developed? My other consideration is less significant--it's more about convenience than compatibility. My target thickness of SU-8 will likely be between 0.5 and 1.0 microns. The lowest thickness listed in Microchem's SU-8 2-25 datasheet (and the SU-8 2000 2-15 datasheet) is 1.5 5m. Would it be practical to achieve sub-micron thicknesses simply with higher spin speeds, or would it be preferable to dilute the SU-8 2 formulation in gamma-butyrolactone (or the SU-8 2002 formulation in cyclopentanone). Does anyone have info on spin speed vs. thickness for dilutions of SU-8 2 (or 2002)? Thanks, -Cyrus