Cyrus,

I am not sure about the entire emission spectra, but I
know that SU-8 is not fluorescent in the red range.
Therefore rhodamine dyes work really well, and the
edge of the SU8 strctures can be seen quite nicely
using DIC.

In order to spin a thinner layer, just thin out the
SU8 in the base solvent.  Microchem sells a thinner of
the base solvent that works nicely.

Jeff

--- Cyrus Wilson  wrote:
> I'm investigating the suitability of SU-8 for
> structures in an
> experimental setup I'm working on, and I have a
> couple of concerns that
> I'd like to look into.
>
> I'm most worried about fluorescence behavior of
> SU-8.  I'd like to be
> able to use fluorescence microscopy to visualize
> fluorescently-labeled
> protein bundles suspended on SU-8 pillars
> (fabricated on a glass cover
> slip).  I have flexibility in my choice of
> fluorophores, so it would
> seem wise to avoid those with excitation frequencies
> in the UV range
> that SU-8 absorbs.  But I'm concerned about slight
> absorption and
> emission of wavelengths above 400nm that aren't a
> problem for most uses
> but could get in my way.  Is anyone aware of info on
> absorption and
> emission spectra of SU-8 after it is baked &
> developed?
>
> My other consideration is less significant--it's
> more about convenience
> than compatibility.  My target thickness of SU-8
> will likely be between
> 0.5 and 1.0 microns.  The lowest thickness listed in
> Microchem's SU-8
> 2-25 datasheet (and the SU-8 2000 2-15 datasheet) is
> 1.5 5m.  Would it
> be practical to achieve sub-micron thicknesses
> simply with higher spin
> speeds, or would it be preferable to dilute the SU-8
> 2 formulation in
> gamma-butyrolactone (or the SU-8 2002 formulation in
> cyclopentanone).
> Does anyone have info on spin speed vs. thickness
> for dilutions of SU-8
> 2 (or 2002)?
>
> Thanks,
> -Cyrus
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