Hi. We are studying DEP microfluidics at the University of Rochester. We are working on an experiment to observe the behavior of water actuated over two nearly parallel vertical electrodes coated with parylene. However, there have been some problems with the adhesion of parylene to steel electrodes. 1. We know the roughness of electrodes will affect the adhesion, but we don't know how smooth we should polish the electrodes, and what steps of cleaning or drying should be performed. 2. We are ready to try A-174 silane as a adhesion promoter, but we don't know some treatment details, for example, how long should the electrodes be kept in the solution, how much time can lapse between applying the adhesion promoter and the parylene coating treatment. Does anybody know something about the above questions or other better methods? We appreciate your suggestion and help!