Hi, I need to bond PDMS to glass, and i have been having problems with this. I have features on the cured PDMS that have to be aligned under the microscope to patterned electrodes (Au/Cr) on a glass slide. Since i need to align the pattern on the cured PDMS to the electrodes, i have a delay of about 2-5 minutes after i have treated both the surfaces with an O2 plasma. I have had better luck when i bond the PDMS with the glass slide immediately after plasma treatment of the two surfaces without allignment. Can anyone suggest a procedure that i can use to obtain fairly good bonding even with the 2-5 min delay after treating the surfaces with an O2 plasma. PDMS curing recipie - 10:1 (sylgard 184 elastomer base: sylgard 184 curing agent) cure the PDMS for 1 hr at 60oC in an oven. Thanks, Varun Reddy