You should be able to bond with cyanoacrylate or applying oxygen plasma to each layer, then pressing the two together to bond. On Thu, Dec 10, 2009 at 10:44 AM, Nano Worldwrote: > Dear all, > > I have a thin layer of PDMS (30µm thick) sandwiched between foils of > polycarbonate. The lower polycarbonate layer has channels of ~25µm depth > and 150µm width. There are interconnects that supplies the fluid to the > channel (water) > > I have to prevent the leakage of water. Hence I am clamping the whole > system > between two thick blocks (by means of screws). When I apply a hydrostatic > pressure I could see the water flow in the micro channels and also in the > interconnects. > > The main problems are: > > (i) If I apply less pressure on the clamping, the water leaks between the > interfaces. > > (ii) If I apply more pressure then there is no flow of water in the > microchannels. I suspect this is due to the fact that the PDMS membrane > stretches and also some form of bending occurs (PDMS might bends and block > the channel) that block the flow of water. > > (iii) The clamping pressure is not uniform and since it is applied manually > this could also have some implications. > > My questions are: > > 1. Is there is any way that I can make the clamping leak proof and in the > same time have a nice flow (less than 1痞/min). > > 2. Lot of bonding methods have been presented in the literature. Has anyone > successfully verified bonding of PDMS/ polycarbonate in their own > laboratory > settings. > > 3. Should a thicker PDMS membrane or a fluid such as isoproponal which has > smaller contact angle with water will solve the problem. > > Thank you in advance > Allwyn. -- Nathan McCorkle Rochester Institute of Technology College of Science, Biotechnology/Bioinformatics