Hi Jian You might try to use an electronic toothbrush, which is available any WalMart. Remove a tip of the toothbrush, and then you will see a metal tip. Give some vibration by tuning on the toothbrush and touch it just on the PDMS surface, which you want to remove bubbles. (It will push bubbles toward outlet of your channels like an ultrasonic pump.) Good Luck Sung ========================================================= Sung Yang Ph.D. Candidate, Bioengineering The Pennsylvania State University, University Park Tel)814-865-6744, 6748 http://biomems.bioe.psu.edu ========================================================= -----Original Message----- From: Xu Jason [mailto:xj_world@hotmail.com] Sent: Thursday, July 01, 2004 9:40 PM To: mems-talk@memsnet.org Subject: [mems-talk] How to get rid of the trapped air bubbles in themicrofluidic chamber? Hello! I am doing some fabrication on the microfluidic chamber by PDMS. When I try to inject the fluid into the chamber, using syringe pump, I always found some air bubbles trapped inside the channel. Does any of you have the experience of handling this case? I tried to tap the PDMS to get rid of them, but it worked not well. Thank you. Sincerely, Jian