Hi, I am working on microfluidic field. recently, I need bonding my device using plasma treatment. I made my device by a PDMS sheet with channel patterns on it and the substrate is a ITO glass where I've made the microelectrodes on it. what I'm confused with is how to bond them together with a good alignment? After bonding I have to drive the fluid into the device using a syringe pump, so it's ideal to bond the device via a plasma treatment. Unfortunately, it require one to put them together immadiately after plasma treatment, but that's barely impossible to align the channels with the ITO microelectrode. Does any one have any experiences on it? thanks a lot. -- 赵亮 南京大学化学化工学院 Liang Zhao School of Chemistry and Chemical Engineering, Nanjing University