You try degassing (place the solution for a *short* period in low pressure, such as in a vacuum desiccator)? - Kevin On 3/17/08 9:46 PM, "Vishwa"wrote: > HI, > > I use 1um fluorescent particles from invitrogen for my microPIV experiments. > I mix the particle solution in nanopure water. The nanopure water is > sonicated for about an hour using Branson 1510 soncator. I then add a bit of > Tween20 to the solution and then mix the particles into this liquid. The > problem is that there are way too many tiny air bubbles in the solution. > Have any one of you faced this problem and what can I do to get rid of this > problem.