Not knowing temperature restrictions, your best bet is to put it under vacuum. If you don't have a vacuum chamber, this can be done using something as simple a food saver vacuum sealer. Best Regards, Mike Sexton EV Group invent * innovate * implement Process Technology Engineer - Direct: +1 (480) 305 2450, Main: +1 (480) 305 2400 Fax: +1 (480) 305 2401 Cell: +1 (602) 625 1275 E-Mail: M.Sexton@EVGroup.com, Web: www.EVGroup.com -----Original Message----- From: mems-talk-bounces@memsnet.org [mailto:mems-talk-bounces@memsnet.org] On Behalf Of Vishwa Sent: Monday, March 17, 2008 1:46 PM To: General MEMS discussion Subject: [mems-talk] Numerous bubbles in microPIV particle solution HI, I use 1um fluorescent particles from invitrogen for my microPIV experiments. I mix the particle solution in nanopure water. The nanopure water is sonicated for about an hour using Branson 1510 soncator. I then add a bit of Tween20 to the solution and then mix the particles into this liquid. The problem is that there are way too many tiny air bubbles in the solution. Have any one of you faced this problem and what can I do to get rid of this problem.